Sreekanth, V. and Bajaj, A. Number of Free Hydroxyl Groups on Bile Acid Phospholipids Determines the Fluidity and Hydration of Model Membranes. Journal of Physical Chemistry B, 17 (40). pp. 12135-12144.

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Abstract

Interactions of synthetic phospholipids with model membranes determines the drug release capabilities of phospholipid vesicles at diseased sites. We performed 1,6-diphenyl-1,3,5-hexatriene (DPH)-based fluorescence anisotropy, Laurdan-based membrane hydration, and differential scanning calorimetry (DSC) studies to cognize the interactions of three bile acid phospholipids, lithocholic acid-phosphocholine (LCA-PC), deoxycholic acid-phosphocholine (DCA-PC), and cholic acid-phosphocholine (CA-PC) with model membranes. These studies revealed that bile acid phospholipids increases membrane fluidity in DCA-PC > CA-PC > LCA-PC order, indicating that induction of membrane fluidity is contingent on the number and positioning of free hydroxyl groups on bile acids. Similarly, DCA-PC causes maximum membrane perturbations due to the presence of a free hydroxyl group, whereas LCA-PC induces gel phase in membranes due to hydrophobic bile acid acyl chain interactions. These DCA-PC-induced membrane perturbations induce a drastic decrease in phase transition temperature (T-m) as determined by calorimetric studies, whereas doping of LCA-PC causes phase transition broadening without change in T-m. Doping of CA-PC induces membrane perturbations and membrane hydration like DCA-PC but sharpening of phase transition at higher doping suggests self-association of CA-PC Molecules. Therefore these differential mode of interactions between bile acid phospholipids and model membranes would help in the future for their use in drug delivery.

Item Type:	Article
Subjects:	Biochemical and Biophysical Sciences
Depositing User:	Unnamed user with email alok@urdip.res.in
Date Deposited:	01 Apr 2015 09:12
Last Modified:	01 Apr 2015 09:12
URI:	http://rcb.sciencecentral.in/id/eprint/10

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