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Nareshkumar, Ambekar and Subbarao, Sindhu and Vennapusa, Amarnatha Reddy and Ashwin, Vargheese and Banarjee, Reema and Kulkarni, Mahesh J. and Ramu, Vemanna S. and Udayakumar, Makarla (2020) Enzymatic and Non-enzymatic Detoxification of Reactive Carbonyl Compounds Improves the Oxidative Stress Tolerance in Cucumber, Tobacco and Rice Seedlings. Journal of Plant Growth Regulation. ISSN 0721-7595

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Detoxifcation of reactive carbonyl compounds (RCC) is crucial to sustain cellular activity to improve plant growth and development. Seedling growth is highly afected by accumulation of RCC under stress. We report non-enzymatic, enzymatic mechanisms of detoxifcation of RCC in the cucumber, tobacco and rice seedling systems exposed to glucose, NaCl, methyl viologen (MV) induced oxidative stress. The cucumber seedlings exposed to carbonyl stress had higher levels of malondialdehyde (MDA), protein carbonyls (PCs) and advanced glycation end-product N-carboxymethyl-lysine (AGE-CML) that negatively afected the seedling growth. The overexpression of enzyme encoding aldo-keto reductase-1 (AKR1) in tobacco and rice showed detoxifcation of RCC, MDA and methylglyoxal (MG) with improved seedling growth under glucose, NaCl and MV-induced oxidative stress. Further, small molecules like acetylsalicylic acid (ASA), aminoguanidine (AG), carnosine (Car), curcumin (Cur) and pyridoxamine (PM) showed detoxifcation of RCC non-enzymatically and rescued the cucumber seedling growth from glucose, NaCl and MV-stress. In autotrophically grown rice seedlings these molecules substantially improved seedling growth under MV-induced oxidative stress. Seedlings treated with the small molecules sustained higher guaiacol peroxidase (GPX) enzyme activity signifying the role of small molecules in reducing carbonyl stress-induced protein inactivation and AGE-CML protein modifcations. The results showed that besides enzymatic detoxifcation of RCC, the small molecules also could reduce cytotoxic efect of RCC under stress. The study demonstrates that small molecules are attractive compounds to improve the seedling growth under stress conditions.

Item Type: Article
Subjects: Biochemical and Biophysical Sciences
Agriculture and Environment
Depositing User: RCB Library
Date Deposited: 18 Feb 2020 10:14
Last Modified: 18 Feb 2020 10:14
URI: http://rcb.sciencecentral.in/id/eprint/173

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