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Agarwal, Megha and Sharma, Akashi and Kumar, Pankaj and Kumar, Amit and Bharadwaj, Anushree and Saini, Masum and Kardon, Gabrielle and Mathew, Sam J. (2020) Myosin heavy chain-embryonic regulates skeletal muscle differentiation during mammalian development. Development, 147 (7). dev184507. ISSN 0950-1991

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Myosin heavy chain-embryonic (MyHC-emb) is a skeletal muscle-specific contractile protein expressed during muscle development. Mutations in MYH3, the gene encoding MyHC-emb, lead to Freeman–Sheldon and Sheldon–Hall congenital contracture syndromes. Here, we characterize the role of MyHC-emb during mammalian development using targeted mouse alleles. Germline loss of MyHC-emb leads to neonatal and postnatal alterations in muscle fiber size, fiber number, fiber type and misregulation of genes involved in muscle differentiation. Deletion of Myh3 during embryonic myogenesis leads to the depletion of the myogenic progenitor cell pool and an increase in the myoblast pool, whereas fetal myogenesis-specific deletion of Myh3 causes the depletion of both myogenic progenitor and myoblast pools. We reveal that the non-cell-autonomous effect of MyHC-emb on myogenic progenitors and myoblasts is mediated by the fibroblast growth factor (FGF) signaling pathway, and exogenous FGF rescues the myogenic differentiation defects upon loss of MyHC-emb function in vitro. Adult Myh3 null mice exhibit scoliosis, a characteristic phenotype exhibited by individuals with Freeman–Sheldon and Sheldon–Hall congenital contracture syndrome. Thus, we have identified MyHC-emb as a crucial myogenic regulator during development, performing dual cell-autonomous and non-cell-autonomous functions.

Item Type: Article
Subjects: Biomedical Science
Biochemical and Biophysical Sciences
Depositing User: RCB Library
Date Deposited: 25 Jun 2020 12:06
Last Modified: 25 Jun 2020 12:06
URI: http://rcb.sciencecentral.in/id/eprint/220

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