Bawa, Simranjot and Brooks, David S and Neville, Kathryn E and Tipping, Marla and Sagar, Md Abdul and Kollhoff, Joseph A and Chawla, Geetanjali and Geisbrecht, Brian V and Tennessen, Jason M and Eliceiri, Kevin W and Geisbrecht, Erika R (2020) Drosophila TRIM32 cooperates with glycolytic enzymes to promote cell growth. eLife, 9. ISSN 2050-084X

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Abstract

Cell growth and/or proliferation may require the reprogramming of metabolic pathways, whereby a switch from oxidative to glycolytic metabolism diverts glycolytic intermediates towards anabolic pathways. Herein, we identify a novel role for TRIM32 in the maintenance of glycolytic flux mediated by biochemical interactions with the glycolytic enzymes Aldolase and Phosphoglycerate mutase. Loss of Drosophila TRIM32, encoded by thin (tn), shows reduced levels of glycolytic intermediates and amino acids. This altered metabolic profile correlates with a reduction in the size of glycolytic larval muscle and brain tissue. Consistent with a role for metabolic intermediates in glycolysis-driven biomass production, dietary amino acid supplementation in tn mutants improves muscle mass. Remarkably, TRIM32 is also required for ectopic growth - loss of TRIM32 in a wing disc-associated tumor model reduces glycolytic metabolism and restricts growth. Overall, our results reveal a novel role for TRIM32 for controlling glycolysis in the context of both normal development and tumor growth.

Item Type:	Article
Subjects:	Biomedical Science Biochemical and Biophysical Sciences
Depositing User:	RCB Library
Date Deposited:	02 Aug 2021 08:11
Last Modified:	02 Aug 2021 08:11
URI:	http://rcb.sciencecentral.in/id/eprint/241

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