Danielová, Adéla and Krejčová, Gabriela and Nedbalová, Pavla and Kazek, Michalina and Strych, Lukáš and Chawla, Geetanjali and Tennessen, Jason M and Lieskovská, Jaroslava and Jindra, Marek and Doležal, Tomáš and Bajgar, Adam (2019) Drosophila macrophages switch to aerobic glycolysis to mount effective antibacterial defense. eLife, 8. ISSN 2050-084X

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Abstract

Macrophage-mediated phagocytosis and cytokine production represent the front lines of resistance to bacterial invaders. A key feature of this pro-inflammatory response in mammals is the complex remodeling of cellular metabolism towards aerobic glycolysis. Although the function of bactericidal macrophages is highly conserved, the metabolic remodeling of insect macrophages remains poorly understood. Here, we used adults of the fruit fly Drosophila melanogaster to investigate the metabolic changes that occur in macrophages during the acute and resolution phases of Streptococcus-induced sepsis. Our studies revealed that orthologs of Hypoxia inducible factor 1α (HIF1α) and Lactate dehydrogenase (LDH) are required for macrophage activation, their bactericidal function, and resistance to infection, thus documenting the conservation of this cellular response between insects and mammals. Further, we show that macrophages employing aerobic glycolysis induce changes in systemic metabolism that are necessary to meet the biosynthetic and energetic demands of their function and resistance to bacterial infection.

Item Type:	Article
Subjects:	Biomedical Science
Depositing User:	RCB Library
Date Deposited:	14 Mar 2023 10:07
Last Modified:	14 Mar 2023 10:07
URI:	http://rcb.sciencecentral.in/id/eprint/262

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