Salunke, Dinakar M. and Vashisht, Sharad and Verma, Sheenam (2019) Cross-clade antibody reactivity may attenuate the ability of influenza virus to evade the immune response. Molecular Immunology, 114. pp. 149-161. ISSN 01615890

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Abstract

Vaccines developed against influenza lose efficacy primarily due to the ability of the virus to generate variantsthat escape recognition of the immune system. Frequent accumulation of mutations in the virus surface proteinsis believed to be responsible for immune evasion. Surprisingly, despite the high mutation rate, the appearance ofnew viral strains through antigenic drift is slow. This delay in the emergence of new strains has been explainedby several different hypotheses over the past decade. In the present study, we have probed the antibody responseagainst multiple clades of influenza neutralizing epitope in the context of antigenic drift. Both, the serum IgGand the monoclonal antibodies raised against the epitope showed strong predisposition against different variantseven with non-conservative mutations. The physiologically relevant binding with hemagglutinin protein and itsvariants revealed multi-reactive recognition potential of human single-chain variable fragments (scFvs).Differential scope for antibody cross-reactivity was evident among different clades that could counterbalance theeffect of antigenic drift. Ourfindings reveal that the majority of epitope variants, which could manifest as singleor double amino acid substitutions, would not escape immune surveillance. However, mutations beneficial forthe virus do appear causing effective antigenic changes. It is suggested that inherent antibody promiscuity couldreduce the deleterious effects of natural mutations on antigen recognition and may be responsible for the delayin the appearance of new antigenic variants of the fast-mutating viruses.

Item Type:	Article
Subjects:	Biochemical and Biophysical Sciences
Depositing User:	RCB Library
Date Deposited:	25 Jun 2020 07:03
Last Modified:	25 Jun 2020 07:03
URI:	http://rcb.sciencecentral.in/id/eprint/269

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