Selokar, Naresh L. and Sharma, Papori and Saini, Monika and Sheoran, Suman and Rajendran, Rasika and Kumar, Dharmendra and Sharma, Rakesh Kumar and Motiani, Rajender K. and Kumar, Pradeep and Jerome, A and Khanna, Sudhir and Yadav, Prem Singh (2019) Successful cloning of a superior buffalo bull. Scientific Reports, 9 (1). ISSN 2045-2322

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Abstract

Somatic cell nuclear transfer (SCNT) technology provides an opportunity to multiply superior animals that could speed up dissemination of favorable genes into the population. In the present study, we attempted to reproduce a superior breeding bull of Murrah buffalo, the best dairy breed of buffalo, using donor cells that were established from tail-skin biopsy and seminal plasma. We studied several parameters such as cell cycle stages, histone modifications (H3K9ac and H3K27me3) and expression of developmental genes in donor cells to determine their SCNT reprogramming potentials. We successfully produced the cloned bull from an embryo that was produced from the skin-derived cell. Growth, blood hematology, plasma biochemistries, and reproductive organs of the produced cloned bull were found normal. Subsequently, the bull was employed for semen production. Semen parameters such as CASA (Computer Assisted Semen Analysis) variables and in vitro fertilizing ability of sperms of the cloned bull were found similar to non-cloned bulls, including the donor bull. At present, we have 12 live healthy progenies that were produced using artificial insemination of frozen semen of the cloned bull, which indicate that the cloned bull is fertile and can be utilized in the buffalo breeding schemes. Taken together, we demonstrate that SCNT can be used to reproduce superior buffalo bulls.

Item Type:	Article
Subjects:	Biomedical Science
Depositing User:	RCB Library
Date Deposited:	25 Jun 2020 06:58
Last Modified:	25 Jun 2020 06:58
URI:	http://rcb.sciencecentral.in/id/eprint/272

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