Sood, Vikas and Sharma, Kiran Bala and Gupta, Vishal and Saha, Dhurjhoti and Dhapola, Parashar and Sharma, Manish and Sen, Utsav and Kitajima, Shigetaka and Chowdhury, Shantanu and Kalia, Manjula and Vrati, Sudhanshu (2017) ATF3 negatively regulates cellular antiviral signaling and autophagy in the absence of type I interferons. Scientific Reports, 7 (1). ISSN 2045-2322

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Abstract

Stringent regulation of antiviral signaling and cellular autophagy is critical for the host response to virus infection. However, little is known how these cellular processes are regulated in the absence of type I interferon signaling. Here, we show that ATF3 is induced following Japanese encephalitis virus (JEV) infection, and regulates cellular antiviral and autophagy pathways in the absence of type I interferons in mouse neuronal cells. We have identifed new targets of ATF3 and show that it binds to the promoter regions of Stat1, Irf9, Isg15 and Atg5 thereby inhibiting cellular antiviral signaling and autophagy. Consistent with these observations, ATF3-depleted cells showed enhanced antiviral responses and induction of robust autophagy. Furthermore, we show that JEV replication was signifcantly reduced in ATF3-depleted cells. Our fndings identify ATF3 as a negative regulator of antiviral signaling and cellular autophagy in mammalian cells, and demonstrate its important role in JEV life cycle.

Item Type:	Article
Subjects:	Biomedical Science
Depositing User:	RCB Library
Date Deposited:	17 Jul 2020 12:10
Last Modified:	17 Jul 2020 12:10
URI:	http://rcb.sciencecentral.in/id/eprint/354

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