Jain, Abha and Kumar, Amit and Shikhi, Meha and Kumar, Ashish and Nair, Deepak T. and Salunke, Dinakar M. (2020) The structure of MP-4 from Mucuna pruriens at 2.22 Å resolution. Acta Crystallographica Section F Structural Biology Communications, 76 (2). pp. 47-57. ISSN 2053-230X

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Abstract

The structure of the MP-4 protein was previously determined at a resolution of 2.8 Å. Owing to the unavailability of gene-sequence information at the time, the side-chain assignment was carried out on the basis of a partial sequence available through Edman degradation, sequence homology to orthologs and electron density. The structure of MP-4 has now been determined at a higher resolution (2.22 Å) in another space group and all of the structural inferences that were presented in the previous report of the structure were validated. In addition, the present data allowed an improved assignment of side chains and enabled further analysis of the MP-4 structure, and the accuracy of the assignment was confirmed by the recently available gene sequence. The study reinforces the traditional concept that conservative interpretations of relatively low-resolution structures remain correct even with the availability of high-resolution data.

Item Type:	Article
Subjects:	Biochemical and Biophysical Sciences
Depositing User:	RCB Library
Date Deposited:	29 Jul 2020 06:40
Last Modified:	29 Jul 2020 06:40
URI:	http://rcb.sciencecentral.in/id/eprint/425

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