Pant, Archana and Bag, Satyabrata and Saha, Bipasa and Verma, Jyoti and Kumar, Pawan and Banerjee, Sayantan and Kumar, Bhoj and Kumar, Yashwant and Desigamani, Anbumani and Maiti, Suhrid and Maiti, Tushar K. and Banerjee, Sanjay K. and Bhadra, Rupak K. and Koley, Hemanta and Dutta, Shanta and Nair, G. Balakrish and Ramamurthy, Thandavarayan and Das, Bhabatosh (2020) Molecular insights into the genome dynamics and interactions between core and acquired genomes of Vibrio cholerae. Proceedings of the National Academy of Sciences. p. 202006283. ISSN 0027-8424

Text Molecular insights into the genome dynamics and interactions between core and acquired genomes of Vibrio cholerae.pdf Restricted to Repository staff only Download (1490Kb) | Request a copy

Abstract

Bacterial species are hosts to horizontally acquired mobile genetic elements (MGEs), which encode virulence, toxin, antimicrobial resistance, and other metabolic functions. The bipartite genome of Vibrio cholerae harbors sporadic and conserved MGEs that contribute in the disease development and survival of the pathogens. For a comprehensive understanding of dynamics of MGEs in the bacterial genome, we engineered the genome of V. cholerae and examined in vitro and in vivo stability of genomic islands (GIs), integrative conjugative elements (ICEs), and prophages. Recombinant vectors carrying the integration module of these GIs, ICE and CTXΦ, helped us to understand the efficiency of integrations of MGEs in the V. cholerae chromosome. We have deleted more than 250 acquired genes from 6 different loci in the V. cholerae chromosome and showed contribution of CTX prophage in the essentiality of SOS response master regulator LexA, which is otherwise not essential for viability in other bacteria, including Escherichia coli In addition, we observed that the core genome-encoded RecA helps CTXΦ to bypass V. cholerae immunity and allow it to replicate in the host bacterium in the presence of similar prophage in the chromosome. Finally, our proteomics analysis reveals the importance of MGEs in modulating the levels of cellular proteome. This study engineered the genome of V. cholerae to remove all of the GIs, ICEs, and prophages and revealed important interactions between core and acquired genomes.

Item Type:	Article
Subjects:	Biochemical and Biophysical Sciences
Depositing User:	RCB Library
Date Deposited:	22 Sep 2020 06:33
Last Modified:	22 Sep 2020 06:33
URI:	http://rcb.sciencecentral.in/id/eprint/530

Actions (login required)

View Item