Ojha, Amrita and Nandi, Dipika and Batra, Harish and Singhal, Rashi and Annarapu, Gowtham K. and Bhattacharyya, Sankar and Seth, Tulika and Dar, Lalit and Medigeshi, Guruprasad R. and Vrati, Sudhanshu and Vikram, Naval K. and Guchhait, Prasenjit (2017) Platelet activation determines the severity of thrombocytopenia in dengue infection. Scientific Reports, 7. p. 41697. ISSN 2045-2322

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Abstract

Thrombocytopenia is common in patients with dengue virus (DENV) infections. With a focus on understanding the possible mechanism of thrombocytopenia in DENV infections we described a direct correlation between activation and depletion of platelets in patients. Our data showed a sharp decrease in platelet counts at day 4 of fever in patients. The high DENV genome copies in platelets correlated directly with the elevated platelet activation along with increased binding of complement factor C3 and IgG on their surface at day 4. Recovery in platelet count was observed on day 10 through day 6 and 8 with simultaneous decrease in platelet activation markers. Further, our in vitro data supported the above observations describing a concentration-dependent increase in platelet activation by DENV serotype-2. The high copy number of DENV2 genome in the platelet pellet correlated directly with platelet activation, microparticle generation and clot formation. Furthermore the DENV2-activated platelets were phagocytosed in large numbers by the monocytes. The DENV2-mediated lysis and clearance of platelets were abrogated in presence of platelet activation inhibitor, prostacyclin. These observations collectively suggest that platelet activation status is an important determinant of thrombocytopenia in dengue infections. A careful strategy of inactivation of platelets may rescue them from rapid destruction during DENV infections.

Item Type:	Article
Subjects:	Biomedical Science Biochemical and Biophysical Sciences
Depositing User:	Dr Vengadesan Krishnan
Date Deposited:	12 Sep 2017 10:01
Last Modified:	30 Aug 2018 11:53
URI:	http://rcb.sciencecentral.in/id/eprint/57

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