Khan, T. and Salunkhe, D. M. Adjustable Locks and Flexible Keys: Plasticity of Epitope-Paratope Interactions in Germline Antibodies. Journal of Immunology, 192 (11). pp. 5398-5405.

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Abstract

Ag recognition by independent primary Abs against a small flexible Ag with overlapping epitopes was analyzed to address the determinants of Ag specificity during the initial encounter. Crystal structures of two distinct dodecapeptide Ags, GDPRPSYISHLL and PPYPAWHAPGNI, in complex with the germline mAb 36-65 were determined and compared with the structures of the same Ags bound to another independent germlinemAb, BBE6.12H3. For each peptideAg, the two germlinem Abs recognized overlapping epitopes, but in different topologies. The peptide structures differed, and the two paratopes attained discrete conformations, leading to different surface topologies, in a mode that can be described as adjustable locks and flexible keys. This is in contrast to mature mAbs, in which conformational convergence of different paratopes while binding to a common epitope in a similar conformation has been reported. These results suggest that the primary immune receptor repertoire is highly versatile as compared with its mature counterpart. Germline and mature mAbs adopt distinct mechanisms for recognizing a flexible epitope. Whereas conservation of conformational repertoire is a key characteristic of mature mAbs achieved through affinity maturation, the germline mAbs, at the initial stages of Ag encounter, maintain substantial plasticity, accommodating a broad specificity repertoire.

Item Type:	Article
Subjects:	Biochemical and Biophysical Sciences
Depositing User:	Unnamed user with email alok@urdip.res.in
Date Deposited:	01 Apr 2015 06:29
Last Modified:	01 Apr 2015 06:29
URI:	http://rcb.sciencecentral.in/id/eprint/6

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