Singh, Deepak and von Ossowski, Ingemar and Palva, Airi and Krishnan, Vengadesan (2013) Purification, crystallization and preliminary crystallographic analysis of the SpaA backbone-pilin subunit from probioticLactobacillus rhamnosusGG. Acta Crystallographica Section F Structural Biology and Crystallization Communications, 69 (10). pp. 1182-1185. ISSN 1744-3091

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Abstract

Lactobacillus rhamnosus GG, a widely used Gram-positive probiotic strain, is clinically well known for its perceived health-promoting effects. It has recently been shown to display proteinaceous pilus fibres (called SpaCBA) on its cell surface. Structurally, SpaCBA pili possess a characteristic three-pilin polymerized architecture, with repeating SpaA major pilins that form the backbone and two types of minor subunits (SpaB and SpaC). In this study, recombinant SpaA protein was purified, characterized and crystallized. The crystals diffracted to a resolution of 2.0 Å and belonged to space group C2, with unit-cell parameters a=227.9, b=63.2, c=104.3 Å, β=95.1°.

Item Type:	Article
Subjects:	Biochemical and Biophysical Sciences
Depositing User:	RCB Library
Date Deposited:	28 Oct 2017 12:14
Last Modified:	28 Oct 2017 12:14
URI:	http://rcb.sciencecentral.in/id/eprint/81

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