Chen, Chen and Krishnan, Vengadesan and Macon, Kevin and Manne, Kartik and Narayana, Sthanam V. L. and Schneewind, Olaf (2013) Secreted Proteases Control Autolysin-mediated Biofilm Growth ofStaphylococcus aureus. Journal of Biological Chemistry, 288 (41). pp. 29440-29452. ISSN 0021-9258

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Abstract

Staphylococcus epidermidis, a commensal of humans, secretes Esp protease to prevent Staphylococcus aureus biofilm formation and colonization. Blocking S. aureus colonization may reduce the incidence of invasive infectious diseases; however, the mechanism whereby Esp disrupts biofilms is unknown. We show here that Esp cleaves autolysin (Atl)-derived murein hydrolases and prevents staphylococcal release of DNA, which serves as extracellular matrix in biofilms. The three-dimensional structure of Esp was revealed by x-ray crystallography and shown to be highly similar to that of S. aureus V8 (SspA). Both atl and sspA are necessary for biofilm formation, and purified SspA cleaves Atl-derived murein hydrolases. Thus, S. aureus biofilms are formed via the controlled secretion and proteolysis of autolysin, and this developmental program appears to be perturbed by the Esp protease of S. epidermidis.

Item Type:	Article
Subjects:	Biomedical Science Biochemical and Biophysical Sciences
Depositing User:	RCB Library
Date Deposited:	28 Oct 2017 12:23
Last Modified:	28 Oct 2017 12:23
URI:	http://rcb.sciencecentral.in/id/eprint/84

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