Sharma, Aashish and Pohane, Amol Arunrao and Bansal, Sandhya and Bajaj, Avinash and Jain, Vikas and Srivastava, Aasheesh (2015) Cell Penetrating Synthetic Antimicrobial Peptides (SAMPs) Exhibiting Potent and Selective Killing ofMycobacteriumby Targeting Its DNA. Chemistry - A European Journal, 21 (9). pp. 3540-3545. ISSN 09476539

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Abstract

Naturally occurring antimicrobial peptides (AMPs) are powerful defence tools to tackle pathogenic microbes. However, limited natural production and high synthetic costs in addition to poor selectivity limit large-scale use of AMPs in clinical settings. Here, we present a series of synthetic AMPs (SAMPs) that exhibit highly selective and potent killing of Mycobacterium (minimum inhibitory concentration <20 μg mL−1) over E. coli or mammalian cells. These SAMPs are active against rapidly multiplying as well as growth saturated Mycobacterium cultures. These SAMPs are not membrane-lytic in nature, and are readily internalized by Mycobacterium and mammalian cells; whereas in E. coli, the lipopolysaccharide layer inhibits their cellular uptake, and hence, their antibacterial action. Upon internalization, these SAMPs interact with the unprotected genomic DNA of mycobacteria, and impede DNA-dependent processes, leading to bacterial cell death.

Item Type:	Article
Subjects:	Biochemical and Biophysical Sciences
Depositing User:	RCB Library
Date Deposited:	15 Nov 2017 12:01
Last Modified:	15 Nov 2017 12:01
URI:	http://rcb.sciencecentral.in/id/eprint/136

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