Verma, Jyoti and Bag, Satyabrata and Saha, Bipasa and Kumar, Pawan and Ghosh, Tarini Shankar and Dayal, Mayanka and Senapati, Tarosi and Mehra, Seema and Dey, Prasanta and Desigamani, Anbumani and Kumar, Dhirendra and Rana, Preety and Kumar, Bhoj and Maiti, Tushar K. and Sharma, Naresh C. and Bhadra, Rupak K. and Mutreja, Ankur and Nair, G. Balakrish and Ramamurthy, Thandavarayan and Das, Bhabatosh (2019) Genomic plasticity associated with antimicrobial resistance in Vibrio cholerae. Proceedings of the National Academy of Sciences, 116 (13). pp. 6226-6231. ISSN 0027-8424

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Abstract

The Bay of Bengal is known as the epicenter for seeding several devastating cholera outbreaks across the globe. Vibrio cholerae, the etiological agent of cholera, has extraordinary competency to acquire exogenous DNA by horizontal gene transfer (HGT) and adapt them into its genome for structuring metabolic processes, developing drug resistance, and colonizing the human intestine. Antimicrobial resistance (AMR) in V. cholerae has become a global concern. However, little is known about the identity of the resistance traits, source of AMR genes, acquisition process, and stability of the genetic elements linked with resistance genes in V. cholerae Here we present details of AMR profiles of 443 V. cholerae strains isolated from the stool samples of diarrheal patients from two regions of India. We sequenced the whole genome of multidrug-resistant (MDR) and extensively drug-resistant (XDR) V. cholerae to identify AMR genes and genomic elements that harbor the resistance traits. Our genomic findings were further confirmed by proteome analysis. We also engineered the genome of V. cholerae to monitor the importance of the autonomously replicating plasmid and core genome in the resistance profile. Our findings provided insights into the genomes of recent cholera isolates and identified several acquired traits including plasmids, transposons, integrative conjugative elements (ICEs), pathogenicity islands (PIs), prophages, and gene cassettes that confer fitness to the pathogen. The knowledge generated from this study would help in better understanding of V. cholerae evolution and management of cholera disease by providing clinical guidance on preferred treatment regimens.

Item Type:	Article
Subjects:	Biomedical Science
Depositing User:	RCB Library
Date Deposited:	25 Jun 2020 07:47
Last Modified:	25 Jun 2020 07:51
URI:	http://rcb.sciencecentral.in/id/eprint/265

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